医院()李云燕
医院卢放根
大同大学医学院侯恒张义平
目的观察嗜酸乳杆菌对溃疡性结肠炎(UC)小鼠结肠黏膜黏液细胞、黏蛋白MUC2的表达，了解其治疗UC的效果及可能作用机制。方法60只BALB/c小鼠随机分为健康对照组、嗜酸乳杆菌（高、低剂量）组、阳性对照（美沙拉嗪）组、阴性对照（冻干赋形剂）组、模型对照组。健康对照组自由饮水，其余各组自由饮用3%葡聚糖硫酸钠7d造模，同时给予嗜酸乳杆菌、美沙拉嗪灌胃治疗。黏液组织化(A学B/PAS)法计数结肠黏液细胞；免疫组织化学检测黏蛋白MUC2的表达抵；采用反转录聚合酶链反应(PCR)检测MUC2的基因表达。结果模型对照组、阴性对照组与健康对照组相比小鼠结肠黏液细胞数、黏蛋M白UC2含量减少，MUC2的基因表达下降；嗜酸乳杆菌和美沙拉嗪不同程度增加结肠黏液细胞数量、黏蛋白含量，促进MUC2的基因表达，嗜酸乳杆菌高剂量组效果较显著。结论嗜酸乳杆菌对急性期UC小鼠有治疗作用，疗效与剂量呈正相关；其机制可能与嗜酸乳杆菌促使结肠黏膜MUC2的表达上调和结肠黏膜中黏液细胞数量增多有关。
结肠炎，溃疡性；益生菌；黏液细胞；粘蛋白；MUC2
TheeffectoflactobacillusonmucouscellinmicewithulcerativecolitisLIYun-yan*，LUFang-gen,HOUHeng,ZHANGYi-pi,ng.*FirstHospi,talofShanxiMedicalUniversity,Taiyuan,China
[Abstract】ObjectiveToobservetheeffectsoflactobacillusonmucouscellsandtheexpressionofmucinMUC2incolonicmucosaofmicewithUlcerativeColitis(UC).TofindtheeffectandpossiblemechanismoftreatingUCwithlactobacillus.MethodsSixtyBALB/cmicewererandomlydividedintonormalcontrol,lactobacillustreatment(highdose,low-da,e),positivecontrol(mesalazine),negativecontrol(excipient),andmodelcontrolgroups.TheexperimentalmiceoftheacutestageofUCwereinducedbydrinking3%dextransulfatesodium(DSS)freelyfor7consecutivedays,andweretreatedwithlactobacillusorMesalazine.AB/PAS,immunohistochemistry,andreversetranscription-polymerasechainreaction(RT-PCR)wereusedtocountthenumber·ofmucouscells,andthe.contentofmucinMUC2andthelevelofMUC2mRNAwastestedrespectively.ResultsThenumberofmucouscellandmucinMUC2(thelevelofproteinandmRNA)werebothdecreasedinmodelcontrolandnegativecontrolgroups